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Blend gently prior to transferring cells to a conical tube made up of 10 mL of pre-warmed wash medium. Take note: Wash the pipette idea and cryovial Along with the wash medium at least three occasions to help strengthen recovery.
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Cross-portion histology followed by hematoxylin and eosin (H&E) staining to evaluate the thickness of the modest or significant airway 가슴수술 epithelium
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Notes: STEMdiff™ Kidney Organoid Package includes a different plate structure/culture established-up and is not a normal monolayer culture; kidney organoids protrude through the underlying monolayer tradition.
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*This is the master elements record with the underneath protocols—all reagents mentioned in Each and every protocol are summarized.
Sure, you’ll discover the step-by-action protocol for TEER measurement To guage the epithelial barrier integrity in ALI cultures here.
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Get rid of supernatant and resuspend cells in FACS buffer. Note: It is important to quench the dissociation reagent by utilizing the 가슴수술 very same or double the volume with the dissociation reagent.